NAD+ & MOTS-c
Cellular energy and metabolic optimization, combined into one ready-to-use injectable. NAD+ restores the coenzyme your mitochondria depend on for energy production and repair; MOTS-c is a mitochondrial-derived peptide that supports metabolic efficiency, insulin sensitivity, and exercise capacity.
Together, they address cellular energy from two directions at once — restoring the raw fuel (NAD+) and improving how efficiently your mitochondria use it (MOTS-c).
The Two Ingredients
NAD+ and MOTS-c both work at the mitochondrial level, but through different mechanisms — one restores the coenzyme your cells run on, the other improves how efficiently your mitochondria produce and use energy.
How These Two Work Together
NAD+ levels decline steadily with age, and that decline is closely tied to reduced cellular energy, slower repair, and diminished metabolic flexibility. MOTS-c works downstream of that — helping the mitochondria you have use available energy more efficiently, particularly under metabolic stress like exercise or caloric restriction. Restoring the fuel supply and improving how it's used address two different points in the same pathway.
Why pair them? NAD+ alone addresses raw cellular fuel supply. MOTS-c alone addresses metabolic efficiency but doesn't replenish NAD+ itself. Used together, clients typically report both the mental clarity and energy associated with NAD+ and the metabolic and exercise-performance benefits associated with MOTS-c — in one injection instead of two separate protocols.
What This Protocol Does For Your Body
Most clients notice mental clarity and steadier energy first, typically within the first week. Metabolic and exercise-capacity changes tend to build over the following 2–4 weeks of consistent use.
NAD+ is a coenzyme required for every mitochondrion in the body to produce ATP. Levels decline significantly with age, and restoring them supports the basic energy currency your cells run on — often felt as steadier daily energy and reduced afternoon fatigue.
MOTS-c signals through pathways involved in glucose uptake and insulin sensitivity, supporting the body's ability to manage blood sugar and use fat as fuel efficiently — particularly relevant during weight management or metabolic optimization protocols.
Brain tissue is highly energy-dependent, and NAD+ repletion is closely associated with improved focus and mental clarity. Many clients describe this as one of the earliest and most noticeable effects of the protocol.
MOTS-c was originally identified for its role in exercise adaptation — supporting mitochondrial biogenesis and helping muscle tissue respond more efficiently to training. Paired with NAD+'s support for cellular repair, this combination is popular with clients building an active training protocol.
NAD+ is a required cofactor for sirtuins and PARP enzymes, both central to DNA repair and cellular maintenance. Adequate NAD+ availability supports these repair processes at the cellular level.
Both NAD+ decline and reduced mitochondrial efficiency are hallmarks of cellular aging. Addressing both simultaneously is why this combination is positioned as a longevity-focused protocol rather than a single-target intervention.
How to Dose NAD+ & MOTS-c
This protocol uses a single fixed dose, three times per week — no titration or guesswork. Your Bespoke Biology vial comes pre-mixed as a liquid, ready to draw and inject. Use the calculator below to confirm your draw if your vial size ever changes.
| What to do | Amount | Details |
|---|---|---|
| Dose per injection | 30 units (0.30 mL) | One fixed dose — draw to the 30-unit mark on your pen injector or syringe |
| Frequency | 3x per week | Non-consecutive days — e.g. Monday, Wednesday, Friday |
| Method | Subcutaneous (SC) | Into the fat just under the skin — not into muscle |
| Injection sites | Abdomen or upper thigh | Rotate sites regularly to avoid irritation |
Using a pen injector? Turn the dial to 30 (0.30 mL). Press the button until the dial returns to "0," then hold the needle in place for 5 seconds before removing to ensure full delivery. Always use a fresh needle for each injection and dispose of used needles in a sharps container.
Slow injection is recommended. NAD+ is well known for causing a warm, flushing sensation if injected too quickly. Administering the dose slowly over 60–90 seconds significantly reduces this effect.
Pen injector step-by-step
- Remove the pen cap and wipe the rubber top with an alcohol pad
- Screw on a new sterile needle
- Turn the dial to 30 units (0.30 mL)
- Clean the injection site with an alcohol pad
- Insert the needle subcutaneously and press the pen button slowly until the dial reaches "0"
- Hold the needle in place for 5 seconds to ensure full delivery
- Remove the needle and dispose of it in a sharps container
Dose Calculator
Your prescribed dose is 30 units (0.30 mL) — the calculator is pre-set to that. If your vial ever changes, update the fields below and the output adjusts automatically.
= 300 mg/mL NAD+ · 2.5 mg/mL MOTS-c
Always confirm the exact concentration printed on your vial label. Because this vial contains two ingredients at different concentrations, per-ingredient amounts are calculated from the ratio above — your provider may give you ingredient-specific guidance that takes priority.
Quick Reference — Your Dose at a Glance
Based on your Bespoke Biology NAD+ & MOTS-c vial: 900 mg NAD+ / 7.5 mg MOTS-c per 3 mL. One dose = 30 units (0.30 mL).
| Vial | Dose | NAD+ per dose | MOTS-c per dose | Shots per vial | Vial duration |
|---|---|---|---|---|---|
| 900 mg / 7.5 mg / 3 mL | 30 units (0.30 mL) | 90 mg | 750 mcg | 10 shots | ~3.3 weeks |
At 3 injections per week, one vial covers approximately 23–24 days of your protocol. Use the calculator above if your vial size ever differs.
Common Questions
Side Effects to Watch For
- /Warmth or flushing during injection — slow administration (60–90 seconds) significantly reduces this
- /Mild fatigue or adjustment tiredness in the first day or two — usually resolves quickly
- /Injection site redness or small bumps — rotate sites every time to prevent this
- /Mild headache when first starting — typically gone within a few days
- /Rare: brief nausea or lightheadedness shortly after injecting — inject more slowly if this happens
Most side effects are mild, related to injection speed, and appear only in the first week or two as your body adjusts. Contact your provider if anything feels unusual or doesn't clear up on its own.
Order NAD+ & MOTS-c
USA-formulated, lab-verified liquid formula. Two mitochondrial-targeted ingredients combined and ready to use — no mixing required. Includes full dosing guidance from our clinical team.
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